Science֪ʳأ㲻һС˽ս
ԹԣʳΪ족ɴ˿ɼԡҪԡ
ΪdzԵ徲սҷع͡ƤЬҡжʳÿ´ѽڣԳгʼۣǵĻȨԡնŵĪҲ̹ԣдij־Ϊӡ
ڡԡƣоעԹ״ǡСĽAgRPԪTHԪPOMCԪʳΪ[1, 2]ǣԡֻһ
Թ״˵ؽʳΪ
һССнںˣTuberal nucleus, TNѧͲ鶯жоද̽оٻᵼʳ֢ں˹ЧҲշݹᾶʣעں˿ʳΪء
ʻߣͼң
20180706գScience־¼оԺYu FuڵҪ[3]ǷںأSomatostatin, SSTԪڽʳΪҪãЩԪͶ䵽ԺˣPVNƴˣBNSTԵؽʳƪ״ηںڼ·еĹЧһչʳơ
Yu Fu
1.óͷСTNSSTԪ
УںˣTNԣLHͷˣTMNԴڱSSTΪǣСжԽںSSTԪTNSSTоǽSST-CreСAi14ӽӴSSTԪɫӫѰףˣǿɿSSTԪеܡ
߷SSTԪڹ״ˣArcTNдڱ棨ͼ1A黯ЧעArcSSTԪAgRPTHPOMCԪ٣ͼ1BTNΧSSTԪLHMCHLHOrxTMNHDCԪ꣨ͼ1CЩԪʳΪл[4, 5]
סʳøǻע伢شóͷСTNSSTԪc-fosˮƽߡĤƬǯʵУ뼢غTNSSTԪͷƵעTNSSTԪ
ͼ1 óͷСTNSSTԪ
2.TNSSTԪؽʳΪ
ʶTNSSTԪ״̬֮ΪоTNSSTԪڽʳΪеijҪԣSST-CreСTNעAAV-DIO-hM3DAAV-DIO-KORDҩＤTNSSTԪշСʳΪڽʳʳһʱ䡢ÿοҧʳʱͽʳƵҩTNSSTԪСʳΪ̭ͨŴѧҪ켤TNSSTԪۣͬͼ2A-N
ΪоTNSSTԪЧĺЧӦSST-CreСTNעAAV-flex-taCasp3յTNSSTԪ10ܺСͼ2O
оע佱շʳΪΪ̽TNSSTԪϲЧӦʹλƫãCPPΪѧʽҩＤTNSSTԪշСƫΪͼ2P
ͼ2 TNSSTԪؽʳΪ
3.TNSSTԪͨPVNBNSTʳΪ
ʶTNSSTԪڽʳΪеijҪ֮߾̽SST-CreСTNעAAV-FLEX-GFPʾЩԪҪΪPVNBNSTCeAPAGPVTPBNͳһȺԪͶ䵽2ArcAgRPġһһͶ䷽[6]ͼ3A-E
Ϊ̽·ؽʳΪSST-CreСTNעAAV-DIO-ChR2PVNBNSTCeAPAGֲˣ⼤TNSST-PVNTNSST-BNST·շʳΪ⼤·Ӱ죨ͼ3F-I
ͼ3 TNSSTԪͨPVNBNSTʳΪ
4.TNSSTԪҪΪGABAԪ
ʶTNSSTԪЧ߾Լϸоͨԭλӽ黯ʵ飬ǷTNSSTԪҪΪ-ᣨGABAԪȰԪͼ4A-D
ΪоTNSSTԪεЧӦʳΪSST-CreСTNעAAV-DIO-hM3DPVNעCNOGABAAɰĵǷѧTNSST-PVN·շʳΪͬʱGABAʹշЧӦͼ4EArcAgRPԪػ[7]עTNSSTԪͨͶʳΪ
ͼ4 TNSSTԪҪΪGABAԪ
ܽ
ʳΪ죬ԡĵĹЧоעArcAgRPԪΪ·Ľ㡣ϵѧŴѧŴѧΪѧҪ죬״η·һ㡪TNSSTԪTNSSTԪշʳΪƻɱTNSSTԪ̭ʳΪӶصģTNSSTԪҪͨPVNBNSTؽʳΪչ˽ʳΪƣڼʳ֪ǸʶԼΪʲô롰ԡ
UG һֱעлشоϣΪоṩ¹ߺоƻٴתͻƣ
οס
1.Gropp, E., et al., Agouti-related peptide-expressing neurons are mandatory for feeding. Nat Neurosci, 2005. 8(10): p. 1289-91.
2.Zhang, X. and A.N. van den Pol, Hypothalamic arcuate nucleus tyrosine hydroxylase neurons play orexigenic role in energy homeostasis. Nat Neurosci, 2016. 19(10): p. 1341-7.
3.Luo, S.X., et al., Regulation of feeding by somatostatin neurons in the tuberal nucleus. Science, 2018.361(6397): p. 76-81.
4.Whiddon, B.B. and R.D. Palmiter, Ablation of neurons expressing melanin-concentrating hormone (MCH) in adult mice improves glucose tolerance independent of MCH signaling. J Neurosci, 2013. 33(5): p. 2009-16.
5.Haas, H. and P. Panula, The role of histamine and the tuberomamillary nucleus in the nervous system. Nat Rev Neurosci, 2003. 4(2): p. 121-30.
6.Betley, J.N., et al., Parallel, redundant circuit organization for homeostatic control of feeding behavior. Cell, 2013. 155(6): p. 1337-50.
7.Garfield, A.S., et al., A neural basis for melanocortin-4 receptor-regulated appetite. Nat Neurosci, 2015. 18(6): p. 863-71.
