Nat Commun | ˣTϸɱTϸѾ
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20227£ϺͨѧѡŶNature Communications“T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies”оʹTϸTϸ壨T cell receptor, TCRβɱԣƺǶϿԭ壨chimeric antigen receptor, CARͨһϵʵ֤ʵһCAR-TϸսԵ徲Ժ
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Tϸֻڻ»ʽϸߣȻḴTϸƴشСӰҪԿٴµսCAR-TϸBϸȪԴİѪʾЧTϸȻCAR-TϸƱлϸԼCAR-TϸɱˣɱTϸΪֹЩ⣬ҪѡϸĿԭ
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02 ںһԭ̼¼Vβ8-CAR-Tϸֳάֺɱ
03 ȱСģ֤Vβ8-CAR-TϸĿЧ
04 Vβ5CAR-Tϸɱ˱;ظ֤ؿսùģ
05 ضVβTϸȱʧ˳ӦߵӰ죬֤CAR-Tս徲
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Ϊ˼CAR-Tϸֳϸɱʹ÷յVβ8+ RajiϸΪԭһظ̼Vβ8-CAR-TϸͨȪԴCAR-Tϸ߷ظĿԭ̼ܹһֱVβ8-CAR-TϸĿΪTϸˢ£ҲûзCD3CD5CAR-Tϸɱ˵ϸԼʵעTϸȣ̼εVβ8-CAR-TϸVβ8+ Jurkatϸɱ
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ʹVβ8е֤ʵVβսú߲֪ڴˣϣùģչTܰϸΪˣ߹Vβ5-CAR-TϸԼVβ5ԵϸCCRF-CEMHut-78 TϸϵVβ8ʵЧһ£Vβ5-CAR-TϸƱУVβ5TϸɨʵУVβ5-CAR-TϸܹɱVβ5+CCRF-CEMHut-78 TϸҽVβ5+ϸʱIFNγͬʵУVβ5-CAR-TϸעܹVβ5+CCRF-CEMֲɣС
VβսȻʾϺõĿЧԱһΣCAR-TϸṥVβ͵Tϸ߽һս徲ԾijضVβ͵TϸռTϸһС֣ƲڰTϸCD5CD7CD3CAR-TսԣһСTϸȱʧᱬصΪ֤һϣ߽ѡȥVβ8+ TϸСģVβ8ԿļעСVβ8+ TϸԼռTϸ21.5%ʹͬscFvƬƱVβ8鿹壬ģVβ8+ TϸȱʧCD3ε崦óͷСΪԱ
߶ԸСģעDEC-OVA磬Vβ8崦óͷСDEC-OVAˮƽԼOT-ITϸӦˮƽҰСƣCD3ε崦óͷСDEC-OVA̭OT-ITϸӦȫ߽һԽB16-OVAϸСп⣬Vβ8+ TϸȱʧСҰСһ£CD3ε崦óͷTϸȱʧСԽѸVβ8+ TϸȱʧСϸҺ߷ӦЩӰ
ʹTCR βɱضΪе㣬ԵCAR-Tϸսԣ֤ʵսܹɱض͵Tϸͬʱֵ˳Ӧ߹Чٴһָ
