Scienceģ˳ӦΣ5-ɫϵͳҪ
۸ǣеֶȻѡЧĸ˳Ӧεǿĸ־Ϳɳ ƣһС˽Ҫռһϯ֮أ˳ӦΧΡ βı䣬ıԼ
ͼƬȪԴ
оעϵͳͶ䣬ӶػӦ˶Ϊ 磺ͰϵͳؼΪ[1]5-ɫϵͳΪ[2]ͷˮƽ5-ɫͣڴΪ[3] ĵǣ5-ɫϵͳСǿӾʽ˦βʽеΪ[4]Ű忴෴
5-ɫṹͼƳɵ
ͼƬȪԴNutrition Wonderland
ڴУ5-ɫҪͷȪԴΪзˣDorsal raphe nucleus, DRN5-ɫܣSerotonergic, 5-HTԪ[5] ȻDRN 5-HTԪǷβı书Чģʽ˶Ϊδ֪
5-ɫϵͳ
ͼƬȪԴNeuron[5]
201921գScience־˿ζѧMelissa WardenڵҪ[6]ǷСٲΣˮƽʱDRN 5-HTԪЧ֮ı䣬˶תΪ˶ ƪ״չ5-ɫϵͳڲе˶ΪĻ·ƣǶ˳Ӧ֪
Melissa Warden
ͼƬȪԴCornell
ȣΪСᳫ˶ʱʵʱDRN 5-HTԪת䣬Sert-CreСDRNעAAV-DIO-GCaMP6sֲˡ ǷڿʵС˶ʼʱDRN 5-HTԪԽͣͼ1A-E
ţСȡˮԴ;ñϰʽϰӵӱܷʽʹСٵλΣͬأǷСڿԽݻӱܵʱᳫ˶DRN 5-HTԪͼ1F-MעСеʱ˶ʼDRN 5-HTԪ
ͼ1С˶ʼDRN 5-HTԪ
2.
˶ʼDRN 5-HTԪ
ȻβʽʹСٸΡ ǣǷÿСʱDRN 5-HTԪߣͼ2A-D ģӱܷʽУСΣʱDRN 5-HTԪ߶ȼͼ2E-Fٴ֤
ϣСеʱ˶ʼDRN 5-HTԪСٸʱ˶ʼDRN 5-HTԪ
ͼ2 ˶ʼDRN 5-HTԪ
3.
й⼤DRN 5-HTԪƻ˶ʼ
ǵ֪DRN 5-HTԪڲеļˮƽȻ෴ ôڲ˹DRN 5-HTԪΪѧЧӦǷͬ෴߾ʹо
Sert-CreСDRNעAAV-DIO-ChR2ֲˣСʽʽӱܷʽУ⼤DRN 5-HTԪС˶Ϊᳫͼ3A-IڸΣβʽУ⼤DRN 5-HTԪС˶Ϊͼ3J-L עУDRN 5-HTԪĹЧ˶תΪ˶
ͼ3 й⼤DRN 5-HTԪƻ˶ʼ
4.
DRN GABAԪС˶ʼ
5-HTԪ֮⣬DRNл-ܣGABAԪΪоDRN GABAԪ˶ΪеĹЧVgat-CreСDRNעAAV-DIO-GCaMP6sֲˣڿʽСԽʱDRN GABAԪƣӱܷʽУСԽʱDRN GABAԪͼ4A-JDRN 5-HTԪΣˮƽ͵мתģʽ תַʽβʽʵЧ֧ۣͬͼ4K-NP-S
ΪоDRN GABAԪˮƽ˶ʼϵVgat-CreСDRNעAAV-DIO-ChR2ֲˣ⼤DRN GABAԪӰתַʽ뿿ʽС˶ʺʹԽݵʱ䣨ͼ4D-EOӱܷʽβʽУ⼤DRN GABAԪС˶ʱ䣨ͼ4I-JT
ЧעDRN GABAԪС˶ʼ
ͼ4 DRN GABAԪС˶ʼ
ģ̭ ֻ˳ӦŻפ о5-ɫϵͳڲʽֲЧ˶˶·ƣ֪֮١ ƪϵƳŴΪѧҪ죬СDRN 5-HTԪ˶ʼЩԪ˶ʼģDRNһԪGABAԪҲС˶ʼ оչ5-ɫϵͳθıģʽĻ·ơ СΣʱ5-ɫϵͳʹʵʱֹضΣʱ5-ɫϵͳʹѸΣգǸõ˳ӦΣ
UG һֱעѧشоϣΪоṩ¹ߺоƻٴתͻƣ
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1. Salamone, J.D., et al., Mesolimbic Dopamine and the Regulation of Motivated Behavior. Curr Top Behav Neurosci, 2016. 27: p. 231-57.
2. Correia, P.A., et al., Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons. Elife, 2017. 6.
3. Marcinkiewcz, C.A., et al., Serotonin engages an anxiety and fear-promoting circuit in the extended amygdala. Nature, 2016. 537(7618): p. 97-101.
4. Warden, M.R., et al., A prefrontal cortex-brainstem neuronal projection that controls response to behavioural challenge. Nature, 2012. 492(7429): p. 428-32.
5. Lesch, K.P. and J. Waider, Serotonin in the modulation of neural plasticity and networks: implications for neurodevelopmental disorders. Neuron, 2012. 76(1): p. 175-91.
6. Green, D.P., et al., A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. Neuron, 2019.
